hamilton microlab star user manual

3UHVVXUH EDVHG PRQLWRULQJ ZRUNV RQO\ ZLWK XQXVHG GLVSRVDEOH WLSV QRW ZLWK QHHGOHV, 7KH YROXPH UDQJH IRU WKH PRQLWRULQJ GHSHQGV RQ WKH VSHFLILF DVVD\ 7KH ORZHU OLPLW LV LQ PDQ\ FDVHV DQ DVSLUDWLRQ YROXPH RI O, 3UHVVXUH EDVHG PRQLWRULQJ KDV EHHQ RSWLPL]HG IRU DTXHRXV VROXWLRQV RQO\. The instrument-specific steps (e.g. STAR stands for Sequential Transfer and Aliquoting Robot. The TCC has four positions for microplates, which are all of the same temperature. Options are defined as components or configurations that are part of the instrument initially provided to the customer as specified by that customer. This is called defining a deck layout. manual_Hamilton_Microlab_STAR_User_Manual Identifier-ark ark:/13960/t6357dz65 Ocr ABBYY FineReader 11.0 (Extended OCR) Page_number_confidence 89.73 Ppi 600 Scanner Internet Archive Python library 1.9.0. plus-circle Add Review. This is a safety precaution, so that channels will not collide with any items that may be on the deck. CO-RE tips are produced under sterile conditions, i.e. Sketch the Cow They are printed in italics in the left margin of the page, begin with the word 'Attention' accompanied by the '!' In this case, aspiration monitoring is deactivated and the tip will follow the falling liquid level (if specified) to the bottom of the container, staying there for the rest of the aspiration. Testing of the Microlab STAR Line is done gravimetrically using a high precision balance. Analysis, Biological Data Measured values are within specifications if less than the values appearing in the table on p. 40. After an aspiration step, the situation within the tip looks like this. Ensure that the voltage selector is correctly set before operating the needle wash station. These methods are then either executed or simulated. This detection approach works for an aspiration with capacitance liquid level detection switched on. System overview and instructions for operating and maintaining the Hamilton ML STAR. The TCC may heat microplates up to 60C, or cool them down to 22C below ambient temperature. The pipetting arm can move in an X direction, whereas each pipetting channel can move relatively independently both in a Y and a Z direction. The deck layout is defined within the Deck Layout Editor, whereas the aspiration and dispense steps are defined using the method editor. Personalised ads and content, ad and content measurement, audience insights and product development. Search the history of over 821 billion Washing starts. One Controller can control up to two vacuum boxes. The Microlab STAR supports pipetting with disposable tips or with needles. The front of the controller has eight ports. Pipetting means transfer of small quantities of liquid from one container to another. We now turn to a description of the components of the automated vacuum box system. A pipetting operation is achieved by aspirating (drawing) liquid from a source container, then transferring and dispensing (dropping) it into a target container. The Hamilton Microlab STAR and the Hudson SOLO robots both use this graphical approach to make their systems approachable to biologists [44, 48]. The Microlab STARs deck is divided into 54 equal tracks (T) for loading carriers with predetermined positions. One unit (Hamilton EasyBlood System) is dedicated to blood fractionation and the other unit (Hamilton Star) is dedicated to handling urine samples. For HMH96 microtitre plate handling, the instrumentation setup requires a CO-RE 96 probe head, and for serial dilutions, an 8-channel array of 1000 L channel pipettes. fresh needles are picked up from the first module for pipetting, used, and then placed back into the same module. The VRDN WLPH is the length of time wash liquid is held within the wash chamber. Simple Aspiration, for all standard cases, Consecutive Aspiration for aspiration with a tip that has already aspirated liquid, and. -The VRDN WLPH is the length of time wash liquid is held within the wash chamber. All other (slave) editors and the run control are then called from the master editor, using easy access shortcut buttons. Lab Equip will contact you for requirements. Illumina innovative sequencing and array technologies are fueling groundbreaking advancements in life science research, translational and consumer genomics, and molecular diagnostics. 3.fresh needles are picked up from the third module for pipetting, used, and then placed back into the same module. For dispensing in a jet, a varying amount of blowout air is used to make sure that all liquid is dispensed from the tip. Hamiltons Microlab STAR is the next generation pipetting workstation. Training in operation of the Microlab STAR and the User Software will be provided by HAMILTON personnel upon initial set-up. Any departure from the procedures given here could lead to erroneous results or malfunction. It consist of a plate carrier with peltier elements on the instrument deck and a service station with a heat exchanger below the bench. The last aliquot of 20 l is discarded into another container or ejected with the tip. HAMILTON has optimized the standard liquid classes with great care to assure the best pipetting accuracy. By contrast with an 8-channel Microlab STAR, the methods for a 16-channel instrument should be set up in such a way that all positions are processed in batches of at least 8 (or better 16, to optimize the pipetting speed) with 8 (or 16) simultaneous aspirations or dispenses at identical x-coordinates (see section 8.9 for more). Multiple units may be integrated and connected via a control box for higher throughput applications. The Automated Vacuum Box System consists of a vacuum box, a vacuum controller and the controlling software. Infrastructure Revolutionize the work in your lab by replacing manual pipetting with the efficiency and accuracy of the Microlab Prep. The Microlab STAR will be verified by a trained HAMILTON technician upon initial set-up, and thereafter at regular intervals for a period of time specified by service agreements. For volume verification in the field, HAMILTON will supply a verification tool, based on gravimetrical measurements (the gold standard of volume verification). Hamilton Microlab STAR Reference Guide PDF(2 MB) Jul 15, 2015. This site uses cookies. For comparison, the hydrostatic pressure of 100 l of water in our standard tip is around 2 mbar. To preserve plastic materials from damage, do not use organic solvents. To guarantee random access to sample carriers, only the inner tube positions should be used. HAMILTON Bonaduz AG, CH-7402 Bonaduz / Switzerland is the manufacturer of the Microlab STAR. HAMILTON recommends that this verification take place four times yearly. Pipetting mode and liquid class represent two independent sets of information which both have to be specified. This window is equipped with a magnetic switch and it is locked during a run. The system measures the conductive signal when the tip leaves the liquid after aspiration. the product has not been operated in accordance with the user manual; the product is not regularly and correctly maintained; the product is not maintained, repaired or modified by a HAMILTON-authorized representative or user; parts other than original HAMILTON parts are used, except for liquid containers such as microtiter plates and tubes; the product or parts thereof have been altered without written authorization from HAMILTON Bonaduz AG; the product is not returned properly packed in the original HAMILTON packaging. Connect them only to an earthed outlet. The screenshots in this publication have been added exclusively for the purpose of illustration. For conductive liquids, capacitive LLD should normally be used. The Microlab STAR will be unpacked and installed and initial set-up performed by a trained HAMILTON technician. from 5 l to 1000 l (for the use of standard tips = 300 l, and high volume tips = 1000 l). The STAR offers higher deck capacity for labware which equates to more SLAS ANSI format positions. Then, the tip leaves the liquid slowly and heads for the vessel to dispense into. File Formats. Worklist Handling with Microsoft Excel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et instructions for sharing your desktop while working with Technical Support. fresh needles are picked up from the second module for pipetting, used, and then placed back into the same module. Steel needles may be picked up directly from the wash station, or from a separate needle rack, which typically is a tip rack on a tip rack carrier with needles instead of tips. The instrument is equipped with a front shield and a hinged transparent window made of plexiglass. The mains plug is on the rear of the pump station (see picture below). Analyzing the shape of the p(t) curve, the system can distinguish the following situations: -Air is aspirated into the tip (because, for example, the cup has not been filled properly). The sample tracker, which generates a database of liquid transfers in the background can be switched on here, too. Hamilton Microlab Star liquid handlers have unique and flexible functionalities such as indepen-dent liquid aspiration/dispense in each channel, and untethered access to and from any given location on the machine deck layout. Once the liquid surface is detected, an additional immersion depth (typically 2mm) is reached to prevent the aspiration of air, and DVSLUDWLRQ starts. Keep your head and hands away from the work surface of the Microlab STAR when it is in operation the pipetting arm and channels move fast and it is possible to sustain an injury. The manual describes both the hardware and software of the Microlab STAR to the extent that a user needs to know them in order to operate the instrument. The wash parameters can be set within the User Software, prior to each wash step. It includes all linked sub-pages as well, which creates a handy reference for everything you need to know about Liquid Handling. In addition to pressure-based clot detection, the ML STAR is equipped with capacitancebased clot detection, too. Finally, to prevent droplet formation, a variable amount of transport air is aspirated. Page 69 and 70: MICROLAB STAR Line Operator's . The needle wash station is a device for the washing and drying of steel needles parallel to the pipetting process. For aspiration, three modes are possible: Simple Aspiration, for all standard cases, Consecutive Aspiration for aspiration with a tip that has already aspirated liquid, and. The vacuum box is compact and a maximum of two can be used in parallel on the Microlab STAR deck with the same controller. The Microlab STAR is controlled by a dedicated user software program which controls all functions for daily work routine, method programming, running methods, and other services. Loading Document. Type & Size. Connect the wash station only to an earthed outlet. Only if instrument is selected within the configuration editor is the additional tab communication settings visible. All tubing and electrical connections are routed through the docking station at the rear of the instrument. However, if the Microlab STAR is subjected to electromagnetic RF fields, or if static electricity is discharged directly onto the Microlab STAR, its Liquid Level Detection ability (see below) may be negatively affected. Contact Us. fresh needles are picked up from the third module for pipetting, used, and then placed back into the same module. To configure the User Software to a specific instrument configuration, the Configuration Editor is available within the Deck Layout Editor under Tools->Configuration: The first decision to be made - prior to a run - is whether to run the method with the instrument or to simulate the run without using the instrument. Page 67 and 68: MICROLAB STAR Line Operator's . The step selection finally allows you to exclude steps such as the move commands from the method editor. Share With Tech Support. This can be achieved e.g. The return string separators are used to define the syntax of single step return values. The number of blank positions to be used for the different instrument configurations is listed in the following table: The 16-channel Microlab STAR is intended as a batch-type processor, i.e., random access to all positions is not possible. Structure Information for Handling the Autoload Unit Information for Special Units. = 3RVLWLRQV RI &DUULHUV 5DFNV DQG &RQWDLQHUV, 5HIHUHQFH *XLGH ([DPSOHV 8VLQJ WKH +6/ 0HWKRG (GLWRU, &UHDWH D 0HWKRG WR &RS\ IURP 3ODWH WR 3ODWH, 1HHGOH :DVK 6WDWLRQ &KHPLFDO &RPSDWLELOLW\. The Hamilton Heater Shaker (HHS) automates the heating and shaking of ANSI/SLAS footprint microplates. You should read through the entire manual before beginning to operate your instrument. iSWAP can be chosen by the customer as an ordering option, but can also be installed later by a service technician at the customers request. Not for use in diagnostic procedures (except as specifically noted). Containers Circular Racks ([DPSOH 'HILQLQJ D 5HFWDQJXODU 5DFN ZLWK &RQWDLQHUV. Conditions are valid only for the HAMILTON verification kit. the tips are RNAseand DNAse-free. reads the barcodes of carriers, tubes, and microtiter plates. Instruments with 4,8, or 16 channels are best operated with carriers, holding microplates and tip racks in landscape orientation (e.g. The Microlab STAR and the computer controlling it may be linked in two different ways: The Microlab STAR automatically recognizes the type of communication in use. When you have worked through this manual, you should be quite well able to operate the Microlab STAR. There should be no containers or tips on the Microlab STAR during transportation. The design specifications mentioned above are valid under the following conditions, obtained for measurements at HAMILTON Bonaduz: Gravimetric testing at Hamilton. Only original HAMILTON Microlab STAR-specific parts and tools may be used with the Microlab STAR, e.g. Teaching mode refers to the possibility of teaching positions on the instrument deck, which can be done moving the arm by the. values). On aspiration, the tip follows the falling liquid level (if so specified) according to the volume aspirated. The values given refer to use of 8 pipetting channels. No contamination or dilution by system liquids. $VSLUDWH D ZDWHU OLNH OLTXLG VLQJOH GLVSHQVH LQWR D SUH ILOOHG ZHOO SODWH XVH ORZ YROXPH WLSV FKDQJH WLSV HYHU\ F\FOH: Surface Dispense Empty Tip (in the liquid class selected here, the, Aspiration: LLD = Pressure or Capacitance or both, Submerge Depth, Dispense: Capacitance LLD on, following liquid level, $VSLUDWH O RI D ZDWHU OLNH OLTXLG GLVSHQVH WKH VDPH DPRXQW LQWR DQ HPSW\, Aspiration: Capacitance LLD, submerge depth 2mm, following liquid, On first aspiration, pre-wetting of the tip by 1-3 mixing cycles is. Always dispense using a residual volume, i.e., do not completely empty the tip on dispense. In the picture on the right, the carriage is on the way to the elution chamber. Additionally, Hamilton pipette channels and tips are designed to fit precisely together using a patented lock-and-key design called CO-RE (Compressed O-Ring Expansion). This creates a tight seal with precise tip alignment to eliminate tip distortion and . The general modules get all the specific information they need about instruments (such as pipetting steps, or information about the probe head) from the instrument-dependent modules. Same commonly accepted pipetting principle as for hand pipettes. Washing starts. The Microlab 600 Basic manual. A user will typically wish to carry out low, medium, or high volume contamination-free pipetting with disposable tips or with steel needles. The units can be reprograamed to aliquot other biological samples as well. Whenever a manual amendment is issued, detailed instructions on amending the existing manual will be provided. Use filter tips to avoid contamination of the pipetting channel by jets, aerosols, etc. To save a method and the corresponding deck layout under a new name: Start the Method Editor as the (first opened) master editor. Better user guidance and programming. The new technology of the Hamilton Microlab STAR liquid handler has unique advantages as a liquid handler desgned to provide the analyst with additional flexibility, such as independent liquid aspiration/dispense in each channel, and untethered access to and from any given location on the machine deck layout. Select the appropriate voltage at the pump station of the needle washer. The aspiration monitoring can be switched on and off for each individual aspiration step of a method using the appropriate commands (see section 2.4). | The scalability of STAR Line instruments means that the widest possible range of throughputs and budgets can be accommodated: additional pipetting channels, a CO-RE 96, 384 Probe Head or an iSWAP plate handling arm can be fitted to existing configurations. Download PDF. The basic model Microlab STAR contains a work surface, called a deck, for placing movable carriers. For pressure-based clot detection, a threshold can be given in arbritrary A/D (analog/digital) values (typically 100 A/D. For example, from the front going to the back, the STAR deck can accommodate 5 microplate or 32 carriers, racks, tips, steel needles, and waste containers. The sensitivity of the capacitive LLD that is to be used depends on the vessel size and the conductivity (or polarity) of the liquid that is to be detected. $FFXUDF\ 7UXHQHVV DQG 3UHFLVLRQ 6SHFLILFDWLRQV. The different barcodes which are allowed on the instrument can be selected here. It is a WindowsTM-based, menu-driven interface allowing the user to define deck layouts and methods, and then to run the Microlab STAR. Always dispense to a surface. The error settings tab allows you to specify the step width in mm for blood clot error recovery (the channels move up on request by the step width). Commercially Surface Dispense Part Volume for dispensing only a part of the liquid in the tip to a surface, leaving a residual volume in the tip. The distance to follow is computed from the known geometry of the (first segment of the) liquid container. Pictured above is the right side of the controller with the different connections. Run execution (for running methods on the instrument). Given that structure, a system with different HAMILTON instruments (even non-HAMILTON instruments, suitably adapted) can be controlled and operated in the same manner, in the same environment and at the same time. There is a presence sensor that identifies the tubes present on a carrier. If working with biohazardous samples, observe and carry out the maintenance procedures paying particular regard to cleaning and decontamination. Typical values are 10-5 to 10-6. symbol, or the word Note, as appropriate. Single Steps . 8VLQJ /LEUDU\ )XQFWLRQV WKH 0/ 67$5 /LEUDU\. The operator of the Microlab STAR must have attended an appropriate training course. 1.5.1 User Manual P/N 610766 The basic reference for the user is the Microlab STAR User Manual. aspiration, dispense - see below) are the same for the. The process is repeated again. The Microlab STAR uses air displacement pipetting to achieve superior measurement accuracy. This manual refers to User Software release 3.0 for the Microlab STAR. Reviews There are no reviews yet. HAMILTON or the authorised HAMILTON representative will repair or replace, at its option and free of charge, any product that under proper and normal use proves to be defective during the warranty period. Independent and modular design of pipetting heads for most flexible assay programming. $Q DERUWHG UXQ VWRSSHG E\ RSHQLQJ WKH IURQW ZLQGRZ FDQQRW EH UHVWDUWHG ,I \RX QHHG WR RSHQ WKH ZLQGRZ GXULQJ D UXQ FOLFN 3DXVH LQ WKH 5XQ 6FUHHQ ZDLW XQWLO WKH LQVWUXPHQW VWRSV DQG WKHQ RSHQ WKH ZLQGRZ. The instruments behavior is determined by specifying the pipetting mode (e.g., surface or jet mode) and the liquid class. If sampling aggressive liquids, use filter tips. This User Manual is designed to help you get the most out of your Microlab STAR. The new method B is still linked to the old deck layout A. Jet Dispense Part Volume for dispensing only a part of the liquid in the tip in a jet, i.e. It is mission critical for us to deliver innovative, flexible, and scalable solutions to meet the needs of our customers. Different tabs are visible: Select your instrument configuration. For dispensing to a liquid surface use cLLD to detect the, position of the surface and then dispense by following the rising liquid level.

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